{"id":27650,"date":"2023-03-20T11:29:32","date_gmt":"2023-03-20T10:29:32","guid":{"rendered":"https:\/\/archive.iask.hu\/hu\/?p=27650&#038;preview=true&#038;preview_id=27650"},"modified":"2023-03-20T11:29:32","modified_gmt":"2023-03-20T10:29:32","slug":"ssr-based-molecular-identification-and-population-structure-analysis-for-forage-pea-pisum-sativum-var-arvense-l-landraces","status":"publish","type":"post","link":"https:\/\/archive.iask.hu\/en\/ssr-based-molecular-identification-and-population-structure-analysis-for-forage-pea-pisum-sativum-var-arvense-l-landraces\/","title":{"rendered":"SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces"},"content":{"rendered":"<p>The article was written with the contribution of <b>P\u00e9ter Poczai<\/b>, iASK researcher and was released in <strong>Genes <\/strong>in 2022.<\/p>\n<p>&nbsp;<\/p>\n<h4>Abstract<\/h4>\n<p>Plant genetic diversity has a significant role in providing traits that can help meet future challenges, such as the need to adapt crops to changing climatic conditions or outbreaks of disease. Our aim in this study was to evaluate the diversity of 61 forage pea specimens (<span class=\"html-italic\">P. sativum<\/span>\u00a0ssp.\u00a0<span class=\"html-italic\">arvense<\/span>\u00a0L.) collected from the northeastern Anatolia region of Turkey using 28 simple sequence repeat (SSR) markers. These primers generated a total of 82 polymorphic bands. The number of observed alleles (Na) per primer varied from 2 to 4 with a mean of 2.89 alleles\/locus. The mean value of expected heterozygosity (Exp-Het = 0.50) was higher than the mean value of observed heterozygosity (Obs-Het =\u20090.22). The mean of polymorphic information content (PIC) was 0.41 with a range of 0.03\u20130.70. The mean number of effective alleles (Ne) was found to be 2.15, Nei\u2019s expected heterozygosity (H) 0.49, and Shannon\u2019s information index (I) 0.81. Cluster analysis through the unweighted pair-group mean average (UPGMA) method revealed that 61 forage pea landraces were divided into three main clusters. Genetic dissimilarity between the genotypes, calculated with the use of NTSYS-pc software, varied between 0.10 (G30 and G34) and 0.66 (G1 and G32). Principal coordinate analysis (PCoA) revealed that three principal coordinates explained 51.54% of the total variation. Moreover, population structure analysis showed that all genotypes formed three sub-populations. Expected heterozygosity values varied between 0.2669 (the first sub-population) and 0.3223 (third sub-population), with an average value of 0.2924. Average population differentiation measurement (Fst) was identified as 0.2351 for the first sub-population, 0.3838 for the second sub-population, and 0.2506 for the third sub-population. In general, current results suggest that SSR markers could be constantly used to illuminate the genetic diversity of forage pea landraces and can potentially be incorporated into future studies that examine the diversity within a larger collection of forage pea genotypes from diverse regions.<\/p>\n<p>&nbsp;<\/p>\n<p><strong>Keywords:<\/strong> genetic, molecular markers, structure, UPGMA<\/p>\n<p>The article is available <a href=\"https:\/\/www.mdpi.com\/2073-4425\/13\/6\/1086\/htm\" target=\"_blank\" rel=\"noopener\" class=\"broken_link\">HERE<\/a>, with full text.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The article was written with the contribution of P\u00e9ter Poczai, iASK researcher and was released in Genes in 2022.<\/p>\n","protected":false},"author":225,"featured_media":27651,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[58,239,97,38,40],"tags":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v16.7 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces - iASK - Institute of Advanced Studies K\u0151szeg<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"http:\/\/archive.iask.hu\/en\/?p=27650\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) Landraces - iASK - Institute of Advanced Studies K\u0151szeg\" \/>\n<meta property=\"og:description\" content=\"The article was written with the contribution of P\u00e9ter Poczai, iASK researcher and was released in Genes in 2022.\" \/>\n<meta property=\"og:url\" content=\"http:\/\/archive.iask.hu\/en\/?p=27650\" \/>\n<meta property=\"og:site_name\" content=\"iASK - Institute of Advanced Studies K\u0151szeg\" \/>\n<meta property=\"article:published_time\" content=\"2023-03-20T10:29:32+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/archive.iask.hu\/wp-content\/uploads\/2002\/07\/karolina.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"1200\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"eszter.takacs@archive.iask.hu\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebSite\",\"@id\":\"https:\/\/archive.iask.hu\/en\/#website\",\"url\":\"https:\/\/archive.iask.hu\/en\/\",\"name\":\"iASK - Institute of Advanced Studies K\\u0151szeg\",\"description\":\"It is the mission of iASK to bring together a critical mass of intellectually open, courageous and knowledge-sharing practitioners \\u2013 those that know that everything is at risk and that conventional research will simply not do, only creative and unusual combinations of ideas.\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\/\/archive.iask.hu\/en\/?s={search_term_string}\"},\"query-input\":\"required name=search_term_string\"}],\"inLanguage\":\"en-US\"},{\"@type\":\"ImageObject\",\"@id\":\"http:\/\/archive.iask.hu\/en\/?p=27650#primaryimage\",\"inLanguage\":\"en-US\",\"url\":\"https:\/\/archive.iask.hu\/wp-content\/uploads\/2002\/07\/karolina.jpg\",\"contentUrl\":\"https:\/\/archive.iask.hu\/wp-content\/uploads\/2002\/07\/karolina.jpg\",\"width\":1200,\"height\":1200},{\"@type\":\"WebPage\",\"@id\":\"http:\/\/archive.iask.hu\/en\/?p=27650#webpage\",\"url\":\"http:\/\/archive.iask.hu\/en\/?p=27650\",\"name\":\"SSR-Based Molecular Identification and Population Structure Analysis for Forage Pea (Pisum sativum var. arvense L.) 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